Cat #: 96-550

Gene Choice® 96-550 Plasmid Midiprep Kit, Up to 250µg, 25 Preps/Unit

Cat #: 96-550

Gene Choice® 96-550 Plasmid Midiprep Kit, Up to 250µg, 25 Preps/Unit

Up to 250µg, Endotoxin-free (<1EU/µg)

25 Preps/Unit

Brand: Gene Choice
  • Purify up to 250µg of endotoxin-free, transfection quality plasmid DNA from 15-50mL overnight culture
  • Fast spin column procedure less than 45 minutes with no gravity flow or alcohol precipitation steps
  • Utilizes syringe filters for rapid bacterial lysate clearance
  • Endotoxin-free (<1EU/µg) plasmid DNA is ready for sensitive downstream applications such as transfection, sequencing, NGS, cloning and PCR
  • Expected yield: 100-250µg for high copy-number; 10-50µg for low copy-number
  • User’s choice of centrifuge or vacuum manifold protocols
Guest Price:
$311.80
List Price: $311.80
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Up to 250µg, Endotoxin-free (<1EU/µg)

25 Preps/Unit

Brand: Gene Choice
  • Purify up to 250µg of endotoxin-free, transfection quality plasmid DNA from 15-50mL overnight culture
  • Fast spin column procedure less than 45 minutes with no gravity flow or alcohol precipitation steps
  • Utilizes syringe filters for rapid bacterial lysate clearance
  • Endotoxin-free (<1EU/µg) plasmid DNA is ready for sensitive downstream applications such as transfection, sequencing, NGS, cloning and PCR
  • Expected yield: 100-250µg for high copy-number; 10-50µg for low copy-number
  • User’s choice of centrifuge or vacuum manifold protocols

The Gene Choice® Plasmid DNA Midiprep Kit from Genesee Scientific isolates up to 250µg of endotoxin-free (<1EU/µg), transfection quality plasmid DNA from 15-50mL overnight culture. The 45-minute spin column procedure allows rapid purification without time-consuming gravity flow or alcohol precipitation steps. After clearing lysate with the provided syringe filters, simply bind-wash-elute to yield endotoxin-free plasmid DNA that is ready for sensitive downstream applications such as transfection, sequencing, NGS, cloning and PCR.

Input 15 - 50 ml plasmid DNA bacterial culture with OD600 between 2 and 3 or equivalent
Plasmid number High-copy, low-copy or cosmid DNA
Processing Time 45 minutes or less
Processing Mode Centrifugation or vacuum manifold
Yield 100 - 250µg for high copy-number & 10 - 50µg for low copy-number
Downstream Applications Transfection, sequencing, NGS, cloning, PCR, etc.

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