Cat #: 11-719
Zymo Research P2005 Strep-Spin Protein Miniprep Kit, Zymo Research, 50 Preps/Unit
Cat #: 11-719
Zymo Research P2005 Strep-Spin Protein Miniprep Kit, Zymo Research, 50 Preps/Unit
Zymo Research
50 Preps/Unit
Brand: Zymo Research- Fast: Purify Strep-tagged proteins from cell lysates in less than 7 minutes
- Simple: Prepare pure protein for small-scale studies using a spin-column
- Convenient: No special instrumentation needed other than a benchtop microcentrifuge
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$404.70
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List Price: $439.30
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Zymo Research
50 Preps/Unit
Brand: Zymo Research- Fast: Purify Strep-tagged proteins from cell lysates in less than 7 minutes
- Simple: Prepare pure protein for small-scale studies using a spin-column
- Convenient: No special instrumentation needed other than a benchtop microcentrifuge
The Strep-Spin Protein Miniprep Kit™ provides a fast spin-column based purification technology for Strep-tagged proteins. Up to 450µg of Strep-tagged protein can be purified in 7 minutes and eluted in Strep-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
| Brand | Zymo Research Corporation |
|---|---|
| Affinity Matrix | Strep-Tactin® XT Superflow® 50% |
| Binding Capacity | 100µl of Strep-Tactin® XT Superflow® 50% suspension can bind 15nmol biotin (approx. 450µg of a 30 kDa Twin-Strep-tag® protein). |
| Elution Method | Biotin excess |
| Elution Volume | Recommended 3 x 200µl |
| Principle of Technology | The Strep-Spin Protein Miniprep Kit is based on a novel StrepTactin® XT Superflow® resin which binds to Twin Strep-tag® with very high affinity. This tag contains two Strep-tag® II peptides connected via a Glycine/Serine linker sequence. Hence this kit is optimized for the purification of Twin-Step®-tagged proteins; however it will also work efficiently for proteins with single Strep-tags. |
| Processing Time | 7 minutes |
| Product Storage | Please store the Strep-Tactin® XT Superflow® 50% suspension, Strep-Wash Buffer, and Strep-Elution Bufer at 4°C. The other components can be stored at room temperature. |
| Protein Purity | Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications. |
| Required Equipment | Microcentrifuge |
| Sample Type | Cell lysates or other complex protein mixtures containing Strep-tagged proteins. |
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